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| Cata #: | Name of Product: | Price: |
| HRP-1057 | Recombinant Human CD18 Protein | $300 |
| Product Name: | Recombinant Human CD18 Protein |
| Catalog #: | HRP-1057 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | The product of human CD18 gene belongs to the integrin b chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin b chain b 2. A given chain may combine with multiple partners resulting in different integrins. For example, b 2 combines with a L chain to form the integrin LFA-1, and combines with the a M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene. Full-length extracellular domain of human CD18 cDNA (23 - 700 aa, derived from BC005861) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (31aa) fusion at its N-terminal and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | CD18 (ITGB; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7) |
| Accession Number: | NP_000202 |
| Species: | Human |
| Package Size: | 50 ug / Vial |
| Composition: | 0.20 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Rosetti,F., et al., Human lupus serum induces neutrophil-mediated organ damage in mice that is enabled by Mac-1 deficiency. J. Immunol. 189 (7), 3714-3723 (2012)
Jawhara,S., et al., Integrin alphaXbeta(2) is a leukocyte receptor for Candida albicans and is essential for protection against fungal infections. J. Immunol. 189 (5), 2468-2477 (2012) Verma,N.K., et al., Leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction induces a novel genetic signature resulting in T-cells refractory to transforming growth factor-beta signaling. J. Biol. Chem. 287 (32), 27204-27216 (2012) |
| Applications: | 1. May be used for in vitro human integrin b chain mediated leukocyte function regulation study with this protein as either coating matrix protein or soluble factor. 2. May be used for protein-protein interaction assay development. 3. As antigen for specific antibody productio |
| Quality Control: | 1. Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHGNLYFQGGEFELQECTKFKVSSCRECIESGPGCTWCQKLNFTGPGDPDSIRCDTRPQLLMRGCAADDIMDPTSLAETQEDHNGGQKQLSPQKVTLYLRPGQAAAFNVTFRRAKGYPIDLYYLMDLSYSMLDDLRNVKKLGGDLLRALNEITESGRIGFGSFVDKTVLPFVNTHPDKLRNPCPNKEKECQPPFAFRHVLKLTNNSNQFQTEVGKQLISGNLDAPEGGLDAMMQVAACPEEIGWRNVTRLLVFATDDGFHFAGDGKLGAILTPNDGRCHLEDNLYKRSNEFDYPSVGQLAHKLAENNIQPIFAVTSRMVKTYEKLTEIIPKSAVGELSEDSSNVVHLIKNAYNKLSSRVFLDHNALPDTLKVTYDSFCSNGVTHRNQPRGDCDGVQINVPITFQVKVTATECIQEQSFVIRALGFTDIVTVQVLPQCECRCRDQSRDRSLCHGKGFLECGICRCDTGYIGKNCECQTQGRSSQELEGSCRKDNNSIICSGLGDCVCGQCLCHTSDVPGKLIYGQYCECDTINCERYNGQVCGGPGRGLCFCGKCRCHPGFEGSACQCERTTEGCLNPRRVECSGRGRCRCNVCECHSGYQLPLCQECPGCPSPCGKYISCAECLKFEKGPFGKNCSAACPGLQLSNNPVKGRTCKERDSEGCWVAYTLEQQDGMDRYLIYVDESRECVAGPN Download Datasheet |