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HTF‐1035 Recombinant Human RB1 Pocket Domain Protein $300

Recombinant Human RB1 Pocket Domain Protein

Product Name: Recombinant Human RB1 Pocket Domain Protein
Catalog #:  HTF‐1035
Manufacture:  LD Biopharma, Inc.

Human retinoblastoma-associated protein (pRB) protein encoded by pRB gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found, promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer and osteogenic sarcoma.

Human RB1 pocket domain cDNA (372 -787 aa), which interact with SV40 T antigen and HPV E7 protein, was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein was expressed in E. coli as inclusion bodies, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  RB1 fragment (OSRC; p105-Pb; pp110; pRb) 
Accession Number:  NP_000312
Species:  Human
Package Size:  20 ug / Vial   
Composition: 0.1 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
Storage: In Liquid. Keep at -20°C for long term storage. Product is stable at 4 °C for at least 7 days.
Key Reference: Daniela Fera, et al., Identification and Characterization of Small Molecule Antagonists of pRb Inactivation by Viral Oncoproteins. Cell, Chemistry & Biology 19, 518-528. (2012)
attman,C.L., et al., Sequential two-step cleavage of the retinoblastoma protein by caspase-3/-7 during etoposide-induced apoptosis. Oncogene 20 (23), 2918-2926 (2001)
Faha,B., et al., Interaction between human cyclin A and adenovirus E1A-associated p107 protein. Science 255 (5040), 87-90 (1992)

1. May be used for in vitro human RB1 mediated oncogenesis regulation study with intracellular protein delivery of this protein.

2. May be used for drug screen assay development with protein-protein interaction ampping such as HPV E7 & RB1 interaction assay development.

3. May be used as antigen for specific antibody production.

Quality Control: Purity: > 90% by SDS-PAGE.
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