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| Cata #: | Name of Product: | Price: |
| HRP-0838 | Recombinant Human RNPS1 Protein | $300 |
| Product Name: | Recombinant Human RNPS1 Protein |
| Catalog #: | HRP-0838 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human RNA-binding protein with serine-rich domain 1 (RNPS1) gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleo-cytoplasmic shuttling protein. Full-length human RNPS1 (305 aa) gene was constructed with 15 aa N-terminal T7 tag and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | RNPS1 (E5.1; SHGC-67834) |
| Accession Number: | NP_006702 |
| Species: | Human |
| Package Size: | 20 ug / Vial |
| Composition: | 0.1 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Li,X., et al., The RNA binding protein RNPS1 alleviates ASF/SF2 depletion-induced genomic instability. RNA 13 (12), 2108-2115 (2007)
Girard,A., et al., A germline-specific class of small RNAs binds mammalian Piwi proteins. Nature 442 (7099), 199-202 (2006) Gehring,N.H., et al., Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements. Mol. Cell 20 (1), 65-75 (2005) |
| Applications: | 1. May be used for in vitro mRNA nuclei-cytoplasmic shuttling regulation study with intracellular delivery of this protein. 2. As soluble / native protein, may be used as enzymatic substrate protein for kinase and ubiquitin assay development. 3. May be used for mapping RNPS1 protein-protein interaction. 4. May be used as antigen for specific antibody development. |
| Quality Control: | 1. Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGEFMDLSGVKKKSLLGVKENNKKSSTRAPSPTKRKDRSDEKSKDRSKDKGATKESSEKDRGRDKTRKRRSASSGSSSTRSRSSSTSSSGSSTSTGSSSGSSSSSASSRSGSSSTSRSSSSSSSSGSPSPSRRRHDNRRRSRSKSKPPKRDEKERKRRSPSPKPTKVHIGRLTRNVTKDHIMEIFSTYGKIKMIDMPVERMHPHLSKGYAYVEFENPDEAEKALKHMDGGQIDGQEITATAVLAPWPRPPPRRFSPPRRMLPPPPMWRRSPPRMRRRSRSPRRRSPVRRRSRSPGRRRHRSRSSSNSSR Download Datasheet |