Invention in the REGENERATIVE MEDICINE is what we do every day, benefits of patients is our final goal

Our Products

Home > Products

Cata #: Name of Product: Price:
HTF-2249 Recombinant Human cJun-11R Protein $300
img

Recombinant Human cJun-11R Protein

Product Name: Recombinant Human cJun-11R Protein
Catalog #:  HTF-2249
Manufacture:  LD Biopharma, Inc.
Intruduction:

Human transcription factor AP-1 (also named as c-Jun) gene encodes a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Recent data indicated that c-Jun overexpression in CAR-T cells induces exhaustion resistance.

             

Full-length human C-Jun cDNA (330aa, derived from BC068522) was constructed with codon optimization using gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal and 11 arginine (11R) tag at its C-terminal. It was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  

C-Jun               ( AP1, c-Jun; p39 )

 
Accession Number:  NP_057953.1
Species:  Human
Package Size:  50 µg / Vial   
Composition: 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose ,DTT and others.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks.
Key Reference:

Rachel C Lynn, et al., C-jun overexpression in CAR-T cells induces exhaustion resistance. Nature. https://doi.org/10.1038/s41586-019-1805-z (2019)

 

Addison WN, et al., Dephosphorylation of the transcriptional cofactor NACA by the PP1A phosphatase enhances cJUN transcriptional activity and osteoblast

 Differentiation. J. Biol. Chem. 294 (20), 8184-8196 (2019)

 

 Yoon H, et al., p27 transcriptionally coregulates cJun to drive programs of tumor progression. Proc. Natl. Acad. Sci. U.S.A. 116 (14), 7005-7014 (2019)

Applications:
  1. May be used for in vitro c-Jun mediated gene transcription regulation study in B cell or neuronal cell’s differentiation by intracellular delivery of this cJun-11R protein directly in vitro cell culture.
  2. May be used for mapping c-Jun protein-protein interaction.
  3. May be used as specific substrate protein for kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays.
  4. Potential biomarker protein / therapeutic target protein for modulating CAR-T cell activities, such as overcome CAR-T cell exhaustion.
  5. As native human c-Jun immunogen for specific antibody production.
Quality Control: Purity: > 92 % by SDS-PAGE.
Recombinant Protein Sequence: MASMTGGQQMGRGHHHHHHENLYFQGGEFTAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDLLTSPDVGLLKLASPELERLIIQSSNGHITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAELHSQNTLPSVTSAAQPVNGAGMVAPAVASVAGGSGSGGFSASLHSEPPVYANLSNFNPGALSSGGGAPSYGAAGLAFPAQPQQQQQPPHHLPQQMPVQHPRLQALKEEPQTVPEMPGETPPLSPIDMESQERIKAERKRMRNRIAASKCRKRKLERIARLEEKVKTLKAQNSELASTANMLREQVAQLKQKVMNHVNSGCQLMLTQQLQTFESGGGGSPGRRRRRRRRRRRDownload Datasheet