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| Cata #: | Name of Product: | Price: |
| HRP-3437 | Recombinant YFP-Human HDAC8 -11R Protein | $300 |
| Product Name: | Recombinant YFP-Human HDAC8 -11R Protein |
| Catalog #: | HRP-3437 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human Histone Deacetylase 8 (HDAC8) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. It also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. HDAC8 may play a role in smooth muscle cell contractility. Full-length human HDAC8 cDNA (376aa, derived from BC050433) was constructed with codon optimization gene synthesis and expressed with YFP Protein as N-terminal (YFP; 256aa) and and 11 arginine (11R) tag at its C-terminal. It was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | HDAC8 (HDACL1; CDA07) |
| Accession Number: | NP_060956.1 |
| Species: | Human |
| Package Size: | 75 µg / Vial |
| Composition: | 1.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT and others. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least two weeks. |
| Key Reference: | Tsai CY, et al., NBM-BMX, an HDAC8 Inhibitor, Overcomes Temozolomide Resistance inGlioblastoma Multiforme by Downregulating thebeta-Catenin /c-Myc/SOX2 Pathway and Upregulating p53-Mediated MGMTInhibition. Int J Mol Sci 22 (11), 5907 (2021) Zhang R, et al., HDAC8-dependent deacetylation of PKM2 directs nuclear localizationand glycolysis to promote proliferation in hepatocellular carcinoma. Cell Death Dis 11 (12), 1036 (2020) Vannini A, et al.,Crystal structure of a eukaryotic zinc-dependent histonedeacetylase, human HDAC8, complexed with a hydroxamic acid Inhibitor. Proc Natl Acad Sci U S A 101 (42), 15064-15069 (2004) |
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| Recombinant Protein Sequence: | MKHHHHHHQVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEFLTAAGITEGMNELYKGSENLYFQGEFEEPEEPADSGQSLVPVYIYSPEYVSMCDSPAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVVESGGGGSPGRRRRRRRRRRR |