Human SHARPIN gene encodes a protein, which is a component of the LUBAC complex that conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex ( TNF-RSC ) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.

Full-length human SHARPIN (325aa, Isoform-II, derived from BC025244) gene was constructed with codon optimization gene synthesis as 29 aa N-terminal T7_His_TEV cleavage site Tag and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Ojo D, et al., A role of SIPL1/SHARPIN in promoting resistance to hormone therapy in breast cancer. Biochim. Biophys. Acta 1864 (3), 735-745 (2018)

 Bal E, et al., Lack of interaction between NEMO and SHARPIN impairs linear ubiquitination and NF-kappaB activation and leads to incontinentia pigmenti. J. Allergy Clin. Immunol. 140 (6), 1671-1682 (2017)

 Liu J, et al., Structural Insights into SHARPIN-Mediated Activation of HOIP for the Linear Ubiquitin Chain Assembly. Cell Rep 21 (1), 27-36 (2017)

• May be used for studying SHARPIN mediated ubiquitin signaling regulation in inflammation pathway, such as NFkb or Wnt by intracellular delivery of recombinant SHARPIN protein using reagent such as ProFectin.
• May be used for mapping SHARPIN protein-protein interaction.
• May be used as specific substrate protein for proteinase related enzyme functional screening assays.
• Highly purified human native SHARPIN antigen, excellent antigen for specific antibody development.