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| Cata #: | Name of Product: | Price: |
| HRP-1987 | Recombinant Human CD69 Protein | $200 |
| Product Name: | Recombinant Human CD69 Protein |
| Catalog #: | HRP-1987 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human CD69 gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of CD69 protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Recent data indicated that myosin light chain 9 & 12 are functional ligands for CD69 that regulate airway inflammation, blocking CD69/myosin light chain 9 & 6 interaction may provide a new strategy for the treatment of asthma. Full-length extracellular domain of human CD69 cDNA (62 – 199 aa) was constructed with codon optimization gene synthesis and expressed with a human Alpha Fetal protein N-terminal-His-TEV cleavage site Tag (217aa) fusion at FAP N-terminal. This protein was expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | CD69 (CLEC2C) |
| Accession Number: | NP_001772 |
| Species: | Human |
| Package Size: | 25 µg / Vial |
| Composition: | 0.25 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Koji Hayashizaki., et al. Myosin light chains 9 & 12 are functional ligands for CD69 that regulate airway inflammation. Sci Immunol. 1, eaaf9154. 16 September (2016). Lin CR., et al. Glycosylation-dependent interaction between CD69 and S100A8/S100A9 complex is required for regulatory T-cell differentiation. FASEB J. 29 (12), 5006-5017 (2015) de la Fuente H, et al., The leukocyte activation receptor CD69 controls T cell differentiation through its interaction with galectin-1. Mol. Cell. Biol. 34 (13), 2479-2487 (2014) Ghafourian M, et al., Increase of CD69, CD161 and CD94 on NK cells in women with recurrent spontaneous abortion and in vitro fertilization failure. Iran J Immunol 11 (2), 84-96 (2014) |
| Applications: | 1. May be used for in vitro CD69 mediated T cell activation regulation study with this protein either as soluble factor or as coating matrix protein. 2. May be used for protein-protein interaction assay. 3. Potential anti- inflammatory response target protein for treatment of various auto-immuno-diseases, such as asthma using CD69 blocking antibodies. 4. As immunogen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSGGSHHHHHHGSENLYFQSVGQYNCPGQYTFSMPSDSHVSSCSEDWVGYQRKCYFISTVKRSWTSAQNACSEHGATLAVIDSEKDMNFLKRYAGREEHWVGLKKEPGHPWKWSNGKEFNNWFNVTGSDKCVFLKNTEVSSMECEKNLYWICNKPYK Download Datasheet |