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| Cata #: | Name of Product: | Price: |
| hRP-0713 | Recombinant Human HAGH Protein | $300 |
| Product Name: | Recombinant Human HAGH Protein |
| Catalog #: | hRP-0713 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Located in mitochondrion, and high level expression in both liver and kidney, human HAGH gene encodes the enzymatic protein which classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Regulation of HAGH activities has been applied in sensitizing cancer cell to various chemotherapy drugs. Full-length human HAGH (14-308 aa) was constructed with 15 N-terminal T7 tag. This protein was expressed in E. coli as inclusion bodies, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | HAGH (GLO2; GLX2; GLXII; HAGH1) |
| Accession Number: | NP_005317 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
| Storage: | In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days. |
| Key Reference: | Antognelli,C., et al., Alteration of glyoxalase genes expression in response to testosterone in LNCaP and PC3 human prostate cancer cells. Cancer Biol. Ther. 6 (12), 1880-1888 (2007) Xu,Y. et al., Glyoxalase II, a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73, is a pro-survival factor of the p53 family. J. Biol. Chem. 281 (36), 26702-26713 (2006) |
| Applications: | 1. May be used for in vitro cellular detoxifying enzyme activity assay development study. 2. May be used for in vitro protein – protein interaction measurement for mapping HAGH binder. 3. May be used as antigen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGEFAALGAACARRGLGPALLGVFCHTDLRKNLTVDEGTMKVEVLPALTDNYMYLVIDDETKEAAIVDPVQPQKVVDAARKHGVKLTTVLTTHHHWDHAGGNEKLVKLESGLKVYGGDDRIGALTHKITHLSTLQVGSLNVKCLATPCHTSGHICYFVSKPGGSEPPAVFTGDTLFVAGCGKFYEGTADEMCKALLEVLGRLPPDTRVYCGHEYTINNLKFARHVEPGNAAIREKLAWAKEKYSIGEPTVPSTLAEEFTYNPFMRVREKTVQQHAGETDPVTTMRAVRREKDQFKMPRD Download Datasheet |