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| Cata #: | Name of Product: | Price: |
| HRP-1503 | Recombinant Human bFGF Protein | $89 |
| Product Name: | Recombinant Human bFGF Protein |
| Catalog #: | HRP-1503 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Fibroblast Growth Factor-basic (bFGF), also known as FGF-2, is a heparin-binding member of the FGF superfamily of molecules. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues by promoting cellular proliferation and differentiation. Additionally, bFGF is a critical component of embryonic stem cell culture medium, allowing cells to remain in an undifferentiated state in serum-free medium. Human mature bFGF is an approximate 17.2 kDa protein consisting of 155 amino acid residues. Full-length mature human bFGF cDNA ( 134 - 288aa ) was constructed with codon optimization as non-fusion protein and expressed in E.coli as soluble protein. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | bFGF (FGF2; HBGF-2) |
| Accession Number: | NP_001997 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | Lyophilized from 5mM Tris-HCl, pH7.5 with 150mM NaCl. |
| Storage: | This product is stable for at least 6 months if stored at -20°C or -80°C. Reconstituted bFGF at a concentration equals to 0.1mg/ml or higher is stable for up to 3 months at -20°C. |
| Key Reference: | Amit,M, et al., Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227: 271-278 (2000).
Xu, C, et al., Basic fibroblast growth factor support undifferentiated human embryonic stem cell growth without conditioned medium. Stem Cells 23: 315-323 (2005) |
| Applications: | 1. May be used as a critical component of human embryonic stem cell in serum free culture medium, allowing hES or iPS cells to remain in an undifferentiated state in chemically defined medium (4 - 10 ng bFGF / ml). 2. Highly purified native protein, can be used ass antigen for specific antibody production. |
| Quality Control: | 1. Purity: > 95% by SDS-PAGE. 2. Endotoxin level is ≤ 1 EU/ug determined by the LAL method. 3. The ED50 is 0.1 to 1.0 ng / ml as determined by the dose dependent proliferation of NIH3T3 cells |
| Recombinant Protein Sequence: | MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS Download Datasheet |