Human Sex comb on Midleg like protein 1 (SCML1) gene spans 18 kb and contains 6 exons. It is transcribed from telomere to centromere. mRNA profiling data indicated that SCML1 express at very low level in most of human adult tissues, but high in fetal / testic tissue. At least 3 Isoforms have been identified for SCML1 gene. The predicted 302-amino acid SCML1 protein contains 2 domains that are present in PcG gene products and their mammalian homologs. In addition to the Scm gene product, the SCML1 protein shares high sequence similarity with the Drosophila polyhomeotic (Ph) gene product, mouse Mph1, and human EDR1 and EDR2, all of which are PcG members or homologs which plays a major role in regulation of gene transcription. SCNL1 can be detected in various cancer cell lines.

Full-length of human SCML1 cDNA (2 – 302aa, Isoform-B, which derived from BC026159) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (31aa) fusion at its N-terminal. This protein was expressed in E. coli as inclusion bodies, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

1. May be used for in vitro mediated gene transcription repressive regulation study in various cancer cells using “ProFectin” based intracellular delivery of this protein.

2. May be used as specific protein substrate for kinase and ubiquitin (Sumo pathway) related enzyme functional screening assays.

3. May be used for SCML1 protein-protein interaction mapping.

4. As immunogen for specific antibody production.