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| Cata #: | Name of Product: | Price: |
| HTF-0075 | Recombinant Human Sox13-11R Protein | $300 |
| Product Name: | Recombinant Human Sox13-11R Protein |
| Catalog #: | HTF-0075 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human Sox13 gene encodes encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. It has also been determined to be a type-1 diabetes autoantigen, also known as islet cell antibody 12. Recombinant human Sox13 protein was constructed with C-terminal tag of 11 arginine domain, which efficiently delivery protein intracellularly. This protein was expressed in E.coli, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Incubating this protein in culture mediums at concentration of 2-8 ug/ml may be used for studying of human HSC or other cell differentiation. |
| Gene Symbol: | Sox13 |
| Accession Number: | NP_005677.2 |
| Species: | Human |
| Package Size: | 50 µg / Vial |
| Composition: | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -20°C for long term storage. Product is stable at 4 °C for at least 7 days. |
| Key Reference: | Marfil,V., et al. Interaction between Hhex and SOX13 modulates Wnt/TCF activity. J. Biol. Chem. 285 (8), 5726-5737 (2010) Hongyan Zhou, et al. Generation of induced pluripotent stem cells using recombinant protein. Cell Stem Cell. Vol 4. Issue 5: 381-384 (2009) |
| Applications: | 1. Protein transduction for human cell differentiation. 2. Active recombinant protein, may be used for ELISA based DNA/Protein binding assay. 3. As specific protein substrate for kinase assay. 4. Immunogen for specific antibody production. |
| Quality Control: | 1. Purity: > 90% by SDS-PAGE. 2.DNA binding activity: to be tested. |
| Recombinant Protein Sequence: | 29aa_Tag_ SMRSPIS AQLALDGVGT MVNCTIKSEE KKEPCHEAPQ GSATAAEPQP GDPARASQDS ADPQAPAQGN FRGSWDCSSP EGNGSPEPKR PGVSEAASGS QEKLDFNRNL KEVVPAIEKL LSSDWKERFL GRNSMEAKDV KGTQESLAEK ELQLLVMIHQ LSTLRDQLLT AHSEQKNMAA MLFEKQQQQM ELARQQQEQI AKQQQQLIQQ QHKINLLQQQ IQQVNMPYVM IPAFPPSHQP LPVTPDSQLA LPIQPIPCKP VEYPLQLLHS PPAPVVKRPG AMATHHPLQE PSQPLNLTAK PKAPELPNTS SSPSLKMSSC VPRPPSHGGP TRDLQSSPPS LPLGFLGEGD AVTKAIQDAR QLLHSHSGAL DGSPNTPFRK DLISLDSSPA KERLEDGCVH PLEEAMLSCD MDGSRHFPES RNSSHIKRPM NAFMVWAKDE RRKILQAFPD MHNSSISKIL GSRWKSMTNQ EKQPYYEEQA RLSRQHLEKY PDYKYKPRPK RTCIVEGKRL RVGEYKALMR TRRQDARQSY VIPPQAGQVQ MSSSDVLYPR AAGMPLAQPL VEHYVPRSLD PNMPVIVNTC SLREEGEGTD DRHSVADGEM YRYSEDEDSE GEEKSDGELV VLTDLEESGG GGSPGRRRRR RRRRRR Download Datasheet |