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| Cata #: | Name of Product: | Price: |
| HTF‐1035 | Recombinant Human RB1 Pocket Domain Protein | $300 |
| Product Name: | Recombinant Human RB1 Pocket Domain Protein |
| Catalog #: | HTF‐1035 |
| Manufacture: | LD Biopharma, Inc. |
| Intruduction: | Human retinoblastoma-associated protein (pRB) protein encoded by pRB gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found, promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer and osteogenic sarcoma. Human RB1 pocket domain cDNA (372 -787 aa), which interact with SV40 T antigen and HPV E7 protein, was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein was expressed in E. coli as inclusion bodies, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. |
| Gene Symbol: | RB1 fragment (OSRC; p105-Pb; pp110; pRb) |
| Accession Number: | NP_000312 |
| Species: | Human |
| Package Size: | 20 ug / Vial |
| Composition: | 0.1 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
| Storage: | In Liquid. Keep at -20°C for long term storage. Product is stable at 4 °C for at least 7 days. |
| Key Reference: | Daniela Fera, et al., Identification and Characterization of Small Molecule Antagonists of pRb Inactivation by Viral Oncoproteins. Cell, Chemistry & Biology 19, 518-528. (2012)
attman,C.L., et al., Sequential two-step cleavage of the retinoblastoma protein by caspase-3/-7 during etoposide-induced apoptosis. Oncogene 20 (23), 2918-2926 (2001) Faha,B., et al., Interaction between human cyclin A and adenovirus E1A-associated p107 protein. Science 255 (5040), 87-90 (1992) |
| Applications: | 1. May be used for in vitro human RB1 mediated oncogenesis regulation study with intracellular protein delivery of this protein. 2. May be used for drug screen assay development with protein-protein interaction ampping such as HPV E7 & RB1 interaction assay development. 3. May be used as antigen for specific antibody production. |
| Quality Control: | Purity: > 90% by SDS-PAGE. |
| Recombinant Protein Sequence: | MASMTGGQQMGRGHHHHHHGNLYFQGGEHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPR Download Datasheet |