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HRP-0837 Recombinant Human PFDN1 Protein $300
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Recombinant Human PFDN1 Protein

Product Name: Recombinant Human PFDN1 Protein
Catalog #:  HRP-0837
Manufacture:  LD Biopharma, Inc.
Intruduction:

Eukaryotic prefoldin (PFD) is a heterohexameric chaperone with a jellyfish-like structure whose function is to deliver nonnative target proteins, principally actins and tubulins, to the eukaryotic cytosolic chaperonin for facilitated folding. Human Prefolding subunit 1 (PFDN1) gene encodes a member of the prefoldin beta subunit family. The encoded PFDN1 protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils.

Full-length human PFDN1 (122 aa) gene was constructed with 15 aa N-terminal T7 tag and expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Gene Symbol:  PFDN1 (PDF; PFD1) 
Accession Number:  NP_002613
Species:  Human
Package Size:  50 µg / Vial   
Composition: 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
Storage: In Liquid. Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.
Key Reference: Gstaiger,M., et al., Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI. Science 302 (5648), 1208-1212 (2003)
Simons,C.T., et al., Selective contribution of eukaryotic prefoldin subunits to actin and tubulin binding. J. Biol. Chem. 279 (6), 4196-4203 (2004)
Applications:

1. May be used for in vitro synthesis protein refolding pathway regulation study with intracellular delivery of this protein.

2. As soluble / native protein, may be used as enzymatic substrate protein for kinase and ubiquitin assay development.

3. May be used for mapping PFDN1 protein-protein interaction. 4. May be used as antigen for specific antibody development.

Quality Control: 1. Purity: > 90% by SDS-PAGE.
Recombinant Protein Sequence: MASMTGGQQMGRGEFMAAPVDLELKKAFTELQAKVIDTQQKVKLADIQIEQLNRTKKHAHLTDTEIMTLVDETNMYEGVGRMFILQSKEAIHSQLLEKQKIAEEKIKELEQKKSYLERSVKEAEDNIREMLMARRAQ
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